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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCT4 Recombinant antibody (84797-4-RR)

    WB analysis using 84797-4-RR (same clone as 84797-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84797-4-RR (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MCT4 Recombinant antibody (84797-4-RR)

    IF Staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84797-4-RR

    Affinity and Kinetic Characterization of 84797-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84797-4-RR against Human MCT4 were performed. The affinity constant is 3.02 nM.

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MCT4 Recombinant antibody, PBS Only

MCT4 Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 84797-4-PBS
Clone No.242296E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, Indirect ELISA

Synonyms

SLC16A3, MCT-4, MCT3, MCT 4, 242296E3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84797-4-PBS targets MCT4 in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCT4 fusion protein Ag18788 相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
Calculated molecular weight 465 aa, 49 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC112269
Gene Symbol MCT4
Gene ID (NCBI) 9123
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15427
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.