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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCTS1 Polyclonal antibody

MCTS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14984-1-AP

Synonyms

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCTS1 Polyclonal antibody (14984-1-AP)

    WB analysis using 14984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14984-1-AP (MCTS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse bladder tissue using MCTS1 Polyclonal antibody (14984-1-AP)

    WB analysis of mouse bladder using 14984-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14984-1-AP (MCTS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using MCTS1 Polyclonal antibody (14984-1-AP)

    IHC staining of human ovarian cancer using 14984-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14984-1-AP (MCTS1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, mouse bladder tissue, K-562 cells, MOLT-4 cells, Raji cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14984-1-AP targets MCTS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCTS1 fusion protein Ag6968 相同性解析による交差性が予測される生物種
Full Name malignant T cell amplified sequence 1
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC001013
Gene symbol MCTS1
Gene ID (NCBI) 28985
RRIDAB_2878096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

Protocols

Product Specific Protocols
WB protocol for MCTS1 antibody 14984-1-APDownload protocol
IHC protocol for MCTS1 antibody 14984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.

Authors - Baiwen Chen
View Article