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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCTS1 Recombinant antibody

MCTS1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241576F2

Cat no : 84312-2-RR

Synonyms

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 241576F2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCTS1 Recombinant antibody (84312-2-RR)

    WB analysis using 84312-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84312-2-RR (MCTS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84312-2-RR

    Affinity and Kinetic Characterization of 84312-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84312-2-RR against Human MCTS1 were performed. The affinity constant is 1.20 nM.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84312-2-RR is MCTS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCTS1 fusion protein Ag6968 相同性解析による交差性が予測される生物種
Full Name malignant T cell amplified sequence 1
Calculated molecular weight 21 kDa
Observed molecular weight18-21 kDa
GenBank accession numberBC001013
Gene symbol MCTS1
Gene ID (NCBI) 28985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

Protocols

Product Specific Protocols
WB protocol for MCTS1 antibody 84312-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols