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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCTS1 Recombinant antibody

MCTS1 Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241576H1

Cat no : 84312-5-RR

Synonyms

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 241576H1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse bladder tissue using MCTS1 Recombinant antibody (84312-5-RR)

    IHC staining of mouse bladder using 84312-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 84312-5-RR (MCTS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using MCTS1 Recombinant antibody (84312-5-RR)

    IHC staining of mouse bladder using 84312-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 84312-5-RR (MCTS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MCTS1 Recombinant antibody (84312-5-RR)

    IF Staining of Jurkat using 84312-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MCTS1 antibody (84312-5-RR, Clone: 241576H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84312-5-RR is MCTS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCTS1 fusion protein Ag6968 相同性解析による交差性が予測される生物種
Full Name malignant T cell amplified sequence 1
Calculated molecular weight 21 kDa
GenBank accession numberBC001013
Gene symbol MCTS1
Gene ID (NCBI) 28985
RRIDAB_3671853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

Protocols

Product Specific Protocols
IHC protocol for MCTS1 antibody 84312-5-RRDownload protocol
IF protocol for MCTS1 antibody 84312-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols