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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDC1 Polyclonal antibody, PBS Only

MDC1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22272-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDC1 Polyclonal antibody (22272-1-AP)

    WB analysis using 22272-1-AP (same clone as 22272-1-PBS)

    Total HEK-293 cells and HEK-293 nuclear extract lysates were subjected to SDS PAGE followed by western blot with 22272-1-AP (MDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22272-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MDC1 Polyclonal antibody (22272-1-AP)

    WB analysis of HeLa using 22272-1-AP (same clone as 22272-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22272-1-AP (MDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22272-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22272-1-PBS targets MDC1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mediator of DNA damage checkpoint 1
Calculated molecular weight 227 kDa
Observed molecular weight~260 kDa
GenBank accession numberNM_014641
Gene symbol MDC1
Gene ID (NCBI) 9656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.