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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDC1 Polyclonal antibody (24721-1-AP)

    WB analysis using 24721-1-AP (same clone as 24721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24721-1-AP (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MDC1 Polyclonal antibody (24721-1-AP)

    IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MDC1 Polyclonal antibody (24721-1-AP)

    IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MDC1 Polyclonal antibody (24721-1-AP)

    IF Staining of HEK-293 using 24721-1-AP (same clone as 24721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MDC1 antibody (24721-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

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MDC1 Polyclonal antibody, PBS Only

MDC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24721-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24721-1-PBS targets MDC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDC1 fusion protein Ag20135 相同性解析による交差性が予測される生物種
Full Name mediator of DNA damage checkpoint 1
Calculated molecular weight 2089 aa, 227 kDa
GenBank accession numberBC152556
Gene Symbol MDC1
Gene ID (NCBI) 9656
RRIDAB_2879688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14676
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.