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  • Featured Product
  • KD/KO Validated

MDH1 Polyclonal antibody

MDH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 15904-1-AP

Synonyms

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MDH1 Polyclonal antibody (15904-1-AP)

    WB analysis of HepG2 using 15904-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MDH1 Polyclonal antibody (15904-1-AP)

    WB analysis of HEK-293 using 15904-1-AP

    WB result of MDH1 antibody (15904-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HL-60 cells using MDH1 Polyclonal antibody (15904-1-AP)

    WB analysis of HL-60 using 15904-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MDH1 Polyclonal antibody (15904-1-AP)

    IP experiment of HepG2 using 15904-1-AP

    IP result of anti-MDH1 (IP:15904-1-AP, 4ug; Detection:15904-1-AP 1:10000) with HepG2 cells lysate 960 ug.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of human renal cell carcinoma using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of human renal cell carcinoma using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of mouse kidney using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of mouse kidney using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of rat heart using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of rat heart using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of human liver cancer using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MDH1 Polyclonal antibody (15904-1-AP)

    IHC staining of human liver cancer using 15904-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MDH1 Polyclonal antibody (15904-1-AP)

    IF Staining of HepG2 using 15904-1-AP

    Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using MDH1 Polyclonal antibody (15904-1-AP)

    FC experiment of HepG2 using 15904-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (15904-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells, HEK-293 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human liver cancer tissue, rat heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15904-1-AP targets MDH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDH1 fusion protein Ag8744 相同性解析による交差性が予測される生物種
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated molecular weight 334 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC001484
Gene symbol MDH1
Gene ID (NCBI) 4190
RRIDAB_2143279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

Protocols

Product Specific Protocols
WB protocol for MDH1 antibody 15904-1-APDownload protocol
IHC protocol for MDH1 antibody 15904-1-APDownload protocol
IF protocol for MDH1 antibody 15904-1-APDownload protocol
IP protocol for MDH1 antibody 15904-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.

Authors - Kıvanç Birsoy
  • KO Validated
View Article
humanWB

Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Authors - Jia-Yuan Zhang
View Article
mouseWB

Blood

MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.

Authors - Hao Gu
View Article
humanWB

J Exp Med

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Authors - Miao Liu
View Article
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
View Article
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
View Article