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KD/KO Validated

Midkine Polyclonal antibody

Name: Midkine Polyclonal antibody
Brand: Proteintech
SKU: 11009-1-AP

Midkine Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11009-1-AP

Synonyms

MDK, Neurite outgrowth-promoting protein, Neurite outgrowth-promoting factor 2, Amphiregulin-associated protein

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11009-1-AP
Mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 11009-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11009-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse embryo using 11009-1-AP
IP result of anti-Midkine (IP:11009-1-AP, 4ug; Detection:11009-1-AP 1:3000) with mouse embryo tissue lysate 4800 ug.
IHC staining of human intrahepatic cholangiocarcinoma using 11009-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11009-1-AP (Midkine antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 11009-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11009-1-AP (Midkine antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11009-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11009-1-AP (Midkine antibody at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11009-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Midkine antibody (11009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11009-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse embryo tissue, COLO 320 cells, SH-SY5Y cells
Positive IP detected in	mouse embryo tissue
Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

11009-1-AP targets Midkine in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Midkine fusion protein Ag1465 相同性解析による交差性が予測される生物種
Full Name	midkine (neurite growth-promoting factor 2)
Calculated molecular weight	16 kDa
Observed molecular weight	16 kDa
GenBank accession number	BC011704
Gene symbol	Midkine
Gene ID (NCBI)	4192
RRID	AB_2250619
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

Protocols

Product Specific Protocols
WB protocol for Midkine antibody 11009-1-AP	Download protocol
IHC protocol for Midkine antibody 11009-1-AP	Download protocol
IF protocol for Midkine antibody 11009-1-AP	Download protocol
IP protocol for Midkine antibody 11009-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Mol Sci Identification of Overexpressed Genes in Malignant Pleural Mesothelioma. Authors - Federica Morani View Article
human	WB,IHC,IF	Front Immunol Roles of the m6A Modification of RNA in the Glioblastoma Microenvironment as Revealed by Single-Cell Analyses. Authors - Feng Yuan KD Validated View Article
human	WB	Front Oncol Targeting MDK Abrogates IFN-γ-Elicited Metastasis inCancers of Various Origins. Authors - Luyu Zheng View Article
human	WB	Cancer Cell Int Midkine promotes glioblastoma progression via PI3K-Akt signaling. Authors - Beiquan Hu KD Validated View Article
		Am J Respir Cell Mol Biol Mesenchymal Glucocorticoid Receptor Regulates Development of Multiple Cell Layers of the Mouse Lung. Authors - Bird A Daniel AD View Article
rat	IHC	J Mol Neurosci Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway. Authors - Ying Xing View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Midkine Polyclonal antibody

Midkine Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11009-1-AP

WB analysis using 11009-1-AP

WB analysis of COLO 320 using 11009-1-AP

IP experiment of mouse embryo using 11009-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 11009-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 11009-1-AP

IHC staining of human liver cancer using 11009-1-AP

IF Staining of HeLa using 11009-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Roles of the m6A Modification of RNA in the Glioblastoma Microenvironment as Revealed by Single-Cell Analyses.

Targeting MDK Abrogates IFN-γ-Elicited Metastasis inCancers of Various Origins.

Midkine promotes glioblastoma progression via PI3K-Akt signaling.

Mesenchymal Glucocorticoid Receptor Regulates Development of Multiple Cell Layers of the Mouse Lung.

Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.