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  • Featured Product
  • KD/KO Validated

MDM1 Polyclonal antibody, PBS Only

MDM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17575-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDM1 Polyclonal antibody (17575-1-AP)

    WB analysis using 17575-1-AP (same clone as 17575-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using MDM1 Polyclonal antibody (17575-1-AP)

    WB analysis of Raji using 17575-1-AP (same clone as 17575-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MDM1 Polyclonal antibody (17575-1-AP)

    IHC staining of human brain using 17575-1-AP (same clone as 17575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MDM1 Polyclonal antibody (17575-1-AP)

    IHC staining of human brain using 17575-1-AP (same clone as 17575-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using MDM1 Polyclonal antibody (17575-1-AP)

    IF Staining of mouse eye using 17575-1-AP (same clone as 17575-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using MDM1 Polyclonal antibody (17575-1-AP)

    IF Staining of mouse eye using 17575-1-AP (same clone as 17575-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using MDM1 Polyclonal antibody (17575-1-AP)

    IF Staining of hTERT-RPE1 using 17575-1-AP (same clone as 17575-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using MDM1 Polyclonal antibody (17575-1-AP)

    IF Staining of hTERT-RPE1 using 17575-1-AP (same clone as 17575-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17575-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17575-1-PBS targets MDM1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDM1 fusion protein Ag10514 相同性解析による交差性が予測される生物種
Full Name Mdm1 nuclear protein homolog (mouse)
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC028355
Gene symbol MDM1
Gene ID (NCBI) 56890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)