• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MDM1 Recombinant antibody (83141-2-RR)

    WB analysis using 83141-2-RR (same clone as 83141-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83141-2-RR (MDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MDM1 Recombinant antibody (83141-2-RR)

    WB analysis of HEK-293 using 83141-2-RR (same clone as 83141-2-PBS)

    WB result of MDM1 antibody (83141-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00166-1

    Sandwich ELISA standard curve of MP00166-1

    Sandwich ELISA standard curve of MP00166-1, Human MDM1 Recombinant Matched Antibody Pair - PBS only. 83141-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10541. 83141-2-PBS was HRP conjugated as the detection antibody. Range: 46.8-3000 pg/mL.

  • Sample test of MP00166-1

    Sample test of MP00166-1

    The mean MDM1 concentration was determined to be 1,326.4 pg/mL in Raji cell extract based on a 4.0 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MDM1 Recombinant antibody (83141-2-RR)

    IHC staining of mouse testis using 83141-2-RR (same clone as 83141-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83141-2-RR (MDM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00166-1

    Cytometric bead array standard curve of MP00166-1

    Cytometric bead array standard curve of MP00166-1, MDM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83141-3-PBS. Detection antibody: 83141-2-PBS. Standard: Ag10541. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00166-2

    Cytometric bead array standard curve of MP00166-2

    Cytometric bead array standard curve of MP00166-2, MDM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83141-1-PBS. Detection antibody: 83141-2-PBS. Standard: Ag10541. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83141-2-RR

    Affinity and Kinetic Characterization of 83141-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83141-2-RR against Human MDM1 were performed. The affinity constant is 3.50 nM.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

MDM1 Recombinant antibody, PBS Only (Detector)

MDM1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83141-2-PBS
Clone No.230425E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Nuclear protein MDM1, 230425E10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83141-2-PBS targets MDM1 as part of a matched antibody pair:

MP00166-1: 83141-3-PBS capture and 83141-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00166-2: 83141-1-PBS capture and 83141-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDM1 fusion protein Ag10541 相同性解析による交差性が予測される生物種
Full Name Mdm1 nuclear protein homolog (mouse)
Calculated molecular weight 81 kDa
Observed molecular weight102 kDa
GenBank accession numberBC028355
Gene Symbol MDM1
Gene ID (NCBI) 56890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TC05
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)