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Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using MDM1 Recombinant antibody (83141-5-RR)

    FC experiment of A431 using 83141-5-RR (same clone as 83141-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Mdm1 Recombinant Antibody (83141-5-RR, Clone:230425E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83141-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83141-5-RR

    Affinity and Kinetic Characterization of 83141-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83141-5-RR against Human MDM1 were performed. The affinity constant is 0.563 nM.

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MDM1 Recombinant antibody, PBS Only

MDM1 Uni-rAbTM Recombinant Antibody for FC (Intra), Indirect ELISA
Cat No. 83141-5-PBS
Clone No.230425E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Synonyms

Nuclear protein MDM1, 230425E2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83141-5-PBS targets MDM1 in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDM1 fusion protein Ag10541 相同性解析による交差性が予測される生物種
Full Name Mdm1 nuclear protein homolog (mouse)
Calculated molecular weight 81 kDa
GenBank accession numberBC028355
Gene Symbol MDM1
Gene ID (NCBI) 56890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8TC05
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mdm1 is highly conserved in mammals as four sorting nexins (Snx): Snx13, 14, 19, and 25. Snx14 alleles are implicated in an autosomal-recessive neurological disease characterized by cellular vacuolization and granular plaque accumulation in late endosomes or autophagosomes. (PMID: 26283797)