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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDM1 Recombinant antibody

MDM1 Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230425E2

Cat no : 83141-5-RR

Synonyms

Nuclear protein MDM1, 230425E2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using MDM1 Recombinant antibody (83141-5-RR)

    FC experiment of A431 using 83141-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Mdm1 Recombinant Antibody (83141-5-RR, Clone:230425E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83141-5-RR

    Affinity and Kinetic Characterization of 83141-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83141-5-RR against Human MDM1 were performed. The affinity constant is 0.563 nM.

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Tested Applications

Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83141-5-RR is MDM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDM1 fusion protein Ag10541 相同性解析による交差性が予測される生物種
Full Name Mdm1 nuclear protein homolog (mouse)
Calculated molecular weight 81 kDa
GenBank accession numberBC028355
Gene symbol MDM1
Gene ID (NCBI) 56890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mdm1 is highly conserved in mammals as four sorting nexins (Snx): Snx13, 14, 19, and 25. Snx14 alleles are implicated in an autosomal-recessive neurological disease characterized by cellular vacuolization and granular plaque accumulation in late endosomes or autophagosomes. (PMID: 26283797)

Protocols

Product Specific Protocols
FC protocol for MDM1 antibody 83141-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols