Featured Product
KD/KO Validated

MDMX/MDM4 Polyclonal antibody

Name: MDMX/MDM4 Polyclonal antibody
Brand: Proteintech
SKU: 17914-1-AP
Rating: 5.00 (2 reviews)

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 17914-1-AP

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of HeLa using 17914-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 17914-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17914-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 17914-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Apoptosised HeLa using 17914-1-AP
Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17914-1-AP
IP result of anti-MDMX (IP:17914-1-AP, 4ug; Detection:17914-1-AP 1:600) with HeLa cells lysate 1120 ug.
IHC staining of human ovary tumor using 17914-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17914-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 17914-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17914-1-AP (MDMX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

-/ -

Cat No. 17914-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 28 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

17914-1-AP targets MDMX/MDM4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MDMX/MDM4 fusion protein Ag12319 相同性解析による交差性が予測される生物種
Full Name	Mdm4 p53 binding protein homolog (mouse)
Calculated molecular weight	490 aa, 55 kDa
Observed molecular weight	80 kDa
GenBank accession number	BC067299
Gene symbol	MDMX/MDM4
Gene ID (NCBI)	4194
RRID	AB_2143683
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MDMX/MDM4 antibody 17914-1-AP	Download protocol
IHC protocol for MDMX/MDM4 antibody 17914-1-AP	Download protocol
IP protocol for MDMX/MDM4 antibody 17914-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IF	Brain Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration. Authors - Hyojung Kim View Article
human	WB	Cancer Res FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX. Authors - Xiang Ling View Article
rat	WB	Redox Biol Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor. Authors - Yuehong Wang View Article
human	WB	Cell Death Discov LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX Authors - Qingqing Zhao View Article
human	WB	J Med Chem Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells. Authors - Shiyan Zhang View Article
mouse	WB	PLoS Genet MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity. Authors - Meenalakshmi Chinnam KO Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Breanna (Verified Customer) (02-18-2020)	It works well Applications: ChIP,
FH Breanna (Verified Customer) (01-29-2020)	Works well Applications: Immunoprecipitation, Flow Cytometry,

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDMX/MDM4 Polyclonal antibody

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 17914-1-AP

WB analysis of A375 using 17914-1-AP

WB analysis of HepG2 using 17914-1-AP

WB analysis of Raji using 17914-1-AP

WB analysis of Apoptosised HeLa using 17914-1-AP

IP experiment of HeLa using 17914-1-AP

IHC staining of human ovary tumor using 17914-1-AP

IHC staining of human ovary tumor using 17914-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.

MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.

Reviews

Related Products

MDM2 Polyclonal antibody