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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDMX/MDM4 Polyclonal antibody

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28747-1-AP

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDMX Polyclonal antibody (28747-1-AP)

    WB analysis using 28747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MDMX Polyclonal antibody (28747-1-AP)

    WB analysis using 28747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using MDMX Polyclonal antibody (28747-1-AP)

    IP experiment of MCF-7 using 28747-1-AP

    IP result of anti-MDMX (IP:28747-1-AP, 4ug; Detection:28747-1-AP 1:3000) with MCF-7 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MDMX Polyclonal antibody (28747-1-AP)

    IHC staining of human ovary tumor using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MDMX Polyclonal antibody (28747-1-AP)

    IHC staining of human breast cancer using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MDMX Polyclonal antibody (28747-1-AP)

    IHC staining of human ovary tumor using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA2780 cells, MCF-7 cells, MDA-MB-231 cells, U2OS cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28747-1-AP targets MDMX/MDM4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDMX/MDM4 fusion protein Ag30456 相同性解析による交差性が予測される生物種
Full Name Mdm4 p53 binding protein homolog (mouse)
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight 70-76 kDa
GenBank accession numberBC067299
Gene symbol MDMX/MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
IHC protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
IP protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

SNRPB2 promotes triple-negative breast cancer progression by controlling alternative splicing of MDM4 pre-mRNA

Authors - Shiyi Yu
View Article