MDMX/MDM4 Recombinant antibody
MDMX/MDM4 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241936D11
Cat no : 84534-5-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293T cells, A549 cells, MCF-7 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 84534-5-RR is MDMX/MDM4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | MDMX/MDM4 fusion protein Ag26678 相同性解析による交差性が予測される生物種 |
| Full Name | Mdm4 p53 binding protein homolog (mouse) |
| Calculated molecular weight | 490 aa, 55 kDa |
| Observed molecular weight | 70-76 kDa |
| GenBank accession number | BC067299 |
| Gene symbol | MDMX/MDM4 |
| Gene ID (NCBI) | 4194 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MDMX/MDM4 antibody 84534-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
