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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDMX/MDM4 Recombinant antibody

MDMX/MDM4 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241936D11

Cat no : 84534-5-RR

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDMX/MDM4 Recombinant antibody (84534-5-RR)

    WB analysis using 84534-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MDMX/MDM4 Recombinant antibody (84534-5-RR)

    IHC staining of human ovary cancer using 84534-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MDMX/MDM4 Recombinant antibody (84534-5-RR)

    IHC staining of human ovary cancer using 84534-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84534-5-RR

    Affinity and Kinetic Characterization of 84534-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84534-5-RR against Human MDMX/MDM4 were performed. The affinity constant is 0.247 nM.

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Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, A549 cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84534-5-RR targets MDMX/MDM4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDMX/MDM4 fusion protein Ag26678 相同性解析による交差性が予測される生物種
Full Name Mdm4 p53 binding protein homolog (mouse)
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight 70-76 kDa
GenBank accession numberBC067299
Gene symbol MDMX/MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MDMX/MDM4 antibody 84534-5-RRDownload protocol
IHC protocol for MDMX/MDM4 antibody 84534-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols