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  • Featured Product
  • KD/KO Validated

ME2 Monoclonal antibody, PBS Only

ME2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E6E2

Cat no : 67457-1-PBS

Synonyms

Malic enzyme 2, ME2, NAD ME

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ME2 Monoclonal antibody (67457-1-Ig)

    WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ME2 Monoclonal antibody (67457-1-Ig)

    WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using ME2 Monoclonal antibody (67457-1-Ig)

    WB analysis of human peripheral blood platelets using 67457-1-Ig (same clone as 67457-1-PBS)

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ME2 Monoclonal antibody (67457-1-Ig)

    WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ME2 Monoclonal antibody (67457-1-Ig)

    WB analysis of HepG2 using 67457-1-Ig (same clone as 67457-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ME2 Monoclonal antibody (67457-1-Ig)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ME2 Monoclonal antibody (67457-1-Ig)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ME2 Monoclonal antibody (67457-1-Ig)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ME2 Monoclonal antibody (67457-1-Ig)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67457-1-PBS targets ME2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ME2 fusion protein Ag20741 相同性解析による交差性が予測される生物種
Full Name malic enzyme 2, NAD(+)-dependent, mitochondrial
Calculated molecular weight 65 kDa
Observed molecular weight 54 kDa, 65 kDa
GenBank accession numberBC000147
Gene symbol ME2
Gene ID (NCBI) 4200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.