Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, mouse lung tissue, mouse brain tissue, rat lung tissue |
| Positive IP detected in | HEK-293T cells, MCF-7 cells |
| Positive IHC detected in | human gliomas tissue, human breast cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 17 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| ChIP | See 5 publications below |
Product Information
10861-1-AP targets MECP2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1309 Product name: Recombinant human MECP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 138-487 aa of BC011612 Sequence: LIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS 相同性解析による交差性が予測される生物種 |
| Full Name | methyl CpG binding protein 2 (Rett syndrome) |
| Calculated molecular weight | 52 kDa, 53 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC011612 |
| Gene Symbol | MECP2 |
| Gene ID (NCBI) | 4204 |
| RRID | AB_2143843 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51608 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
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Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IF protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IHC protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IP protocol for MECP2 antibody 10861-1-AP | Download protocol |
| WB protocol for MECP2 antibody 10861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion. | ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
| ||
Redox Biol Design of anti-depressant phosphodiester amino acidic Keap1-Nrf2 protein-protein interaction inhibitors | ||
Genes Dis PDK1 promotes breast cancer progression by enhancing the stability and transcriptional activity of HIF-1α | ||
Int J Biol Macromol SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo. | ||
Clin Transl Med Downregulated SPESP1-driven fibroblast senescence decreases wound healing in aged mice |