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  • Featured Product
  • KD/KO Validated

MECR Polyclonal antibody

MECR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14932-1-AP

Synonyms

HsNrbf 1, FASN2B, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104, CGI-63

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using MECR Polyclonal antibody (14932-1-AP)

    WB analysis of mouse heart using 14932-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14932-1-AP (MECR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using MECR Polyclonal antibody (14932-1-AP)

    WB analysis of human stomach using 14932-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14932-1-AP (MECR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using MECR Polyclonal antibody (14932-1-AP)

    IHC staining of human heart using 14932-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14932-1-AP (MECR Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using MECR Polyclonal antibody (14932-1-AP)

    IF Staining of C2C12 using 14932-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MECR antibody (14932-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using MECR Polyclonal antibody (14932-1-AP)

    IF Staining of LNCaP using 14932-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using 14932-1-AP (MECR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, human stomach tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14932-1-AP targets MECR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MECR fusion protein Ag6738 相同性解析による交差性が予測される生物種
Full Name mitochondrial trans-2-enoyl-CoA reductase
Calculated molecular weight 40 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001419
Gene symbol MECR
Gene ID (NCBI) 51102
RRIDAB_2235303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MECR antibody 14932-1-APDownload protocol
IHC protocol for MECR antibody 14932-1-APDownload protocol
IF protocol for MECR antibody 14932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,pigWB

PLoS Biol

Alternative splicing liberates a cryptic cytoplasmic isoform of mitochondrial MECR that antagonizes influenza virus

Authors - Steven F Baker
  • KO Validated
View Article