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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MECR Polyclonal antibody (51025-2-Ig)

    WB analysis of mouse skeletal muscle using 51025-2-Ig (same clone as 51025-2-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (MECR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using MECR Polyclonal antibody (51025-2-Ig)

    WB analysis of mouse heart using 51025-2-Ig (same clone as 51025-2-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (MECR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MECR Polyclonal antibody (51025-2-Ig)

    IHC staining of mouse skeletal muscle using 51025-2-Ig (same clone as 51025-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MECR Polyclonal antibody (51025-2-Ig)

    IHC staining of mouse skeletal muscle using 51025-2-Ig (same clone as 51025-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Mouse MECR Polyclonal antibody, PBS Only

MECR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 51025-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51025-2-PBS targets MECR in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitochondrial trans-2-enoyl-CoA reductase
Calculated molecular weight 40 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC003864
Gene Symbol MECR
Gene ID (NCBI) 26922
RRIDAB_1167469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9DCS3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)