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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using MED10 Polyclonal antibody (15196-1-AP)

    WB analysis of COLO 320 using 15196-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15196-1-AP (MED10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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MED10 Polyclonal antibody

MED10 Polyclonal Antibody for WB, ELISA
Cat No. 15196-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

L6, MED10, mediator complex subunit 10, NUT2, TRG 17, TRG 20, TRG17, TRG20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15196-1-AP targets MED10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED10 fusion protein Ag7336 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 10
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC003353
Gene Symbol MED10
Gene ID (NCBI) 84246
RRIDAB_2878117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTT4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED10, also named as L6, TRG17, or TRG20, is a 135 amino acid protein, which belongs to the Mediator complex subunit 10 family. MED10 localizes in the nucleus and as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Multiprotein mediator complex acting as a coactivator is required for activation of RNA polymerase II transcription by DNA bound transcription factors.

Protocols

Product Specific Protocols
WB protocol for MED10 antibody 15196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.

Authors - Vijayalingam S S
View Article