MED10 Polyclonal antibody
MED10 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 15196-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15196-1-AP targets MED10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MED10 fusion protein Ag7336 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 10 |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC003353 |
| Gene symbol | MED10 |
| Gene ID (NCBI) | 84246 |
| RRID | AB_2878117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED10, also named as L6, TRG17, or TRG20, is a 135 amino acid protein, which belongs to the Mediator complex subunit 10 family. MED10 localizes in the nucleus and as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Multiprotein mediator complex acting as a coactivator is required for activation of RNA polymerase II transcription by DNA bound transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED10 antibody 15196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH SITING (Verified Customer) (04-02-2021) | can see band aroun 15kd
 |
