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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using MED12L Polyclonal antibody (20047-1-AP)

    WB analysis of mouse liver using 20047-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20047-1-AP (MED12L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MED12L Polyclonal antibody

MED12L Polyclonal Antibody for WB, ELISA
Cat No. 20047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

TNRC11L, Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20047-1-AP targets MED12L in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 12-like
Calculated molecular weight 240 kDa
Observed molecular weight 260-280 kDa
GenBank accession numberNM_053002
Gene Symbol MED12L
Gene ID (NCBI) 116931
RRIDAB_10734594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The antibody can recognize all the isoform of MED12L. The antibody has no cross reaction to MED12.

Protocols

Product Specific Protocols
WB protocol for MED12L antibody 20047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols