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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MED12L Recombinant antibody

MED12L Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240101C11

Cat no : 83540-1-RR

Synonyms

Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein, KIAA1635

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using MED12L Recombinant antibody (83540-1-RR)

    IHC staining of mouse brain using 83540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83540-1-RR (MED12L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MED12L Recombinant antibody (83540-1-RR)

    IHC staining of mouse brain using 83540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83540-1-RR (MED12L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MED12L Recombinant antibody (83540-1-RR)

    IF Staining of HepG2 using 83540-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED12L antibody (83540-1-RR, Clone: 240101C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using MED12L Recombinant antibody (83540-1-RR)

    FC experiment of U2OS using 83540-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug MED12L Recombinant antibody (83540-1-RR, Clone:240101C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HepG2 cells using MED12L Recombinant antibody (83540-1-RR)

    FC experiment of HepG2 using 83540-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MED12L Recombinant antibody (83540-1-RR, Clone:240101C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83540-1-RR is MED12L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED12L fusion protein Ag34636 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 12-like
Calculated molecular weight240 kDa
GenBank accession numberNM_053002
Gene symbol MED12L
Gene ID (NCBI) 116931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody can recognize all the isoform of MED12L. This antibody has no cross-reaction to MED12.

Protocols

Product Specific Protocols
IHC protocol for MED12L antibody 83540-1-RRDownload protocol
IF protocol for MED12L antibody 83540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols