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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED13-Specific Polyclonal antibody (20029-1-AP)

    WB analysis using 20029-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20029-1-AP (MED13-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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MED13-Specific Polyclonal antibody

MED13-Specific Polyclonal Antibody for WB, ELISA
Cat No. 20029-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ARC250, DRIP250, HSPC221, KIAA0593, MED13, MED13-Specific, mediator complex subunit 13, THRAP1, TRAP240

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20029-1-AP targets MED13-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 13
Calculated molecular weight 239 kDa
Observed molecular weight240-250 kDa
GenBank accession numberNM_005121
Gene Symbol MED13
Gene ID (NCBI) 9969
RRIDAB_3085595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED13, also named as ARC250, KIAA0593, THRAP1 and TRAP240, belongs to the Mediator complex subunit 13 family. MED13 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody is specific to MED13. It has no cross reaction to MED13L.

Protocols

Product Specific Protocols
WB protocol for MED13-Specific antibody 20029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols