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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED23 Polyclonal antibody (27850-1-AP)

    WB analysis using 27850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27850-1-AP (MED23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MED23 Polyclonal antibody (27850-1-AP)

    WB analysis of A375 using 27850-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27850-1-AP (MED23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MED23 Polyclonal antibody (27850-1-AP)

    IHC staining of human stomach using 27850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27850-1-AP (MED23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MED23 Polyclonal antibody

MED23 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27850-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

MED23/CRSP3, CRSP133, CRSP130, CRSP complex subunit 3, Cofactor required for Sp1 transcriptional activation subunit 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, A375 cells, U2OS cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27850-1-AP targets MED23 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED23 fusion protein Ag27241 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 23
Calculated molecular weight 156 kDa
Observed molecular weight 140 kDa, 50 kDa
GenBank accession numberBC060759
Gene Symbol MED23
Gene ID (NCBI) 9439
RRIDAB_2880994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

Protocols

Product Specific Protocols
WB protocol for MED23 antibody 27850-1-APDownload protocol
IHC protocol for MED23 antibody 27850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Authors - Xu Wang
View Article