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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED30 Monoclonal antibody (67038-1-Ig)

    WB analysis using 67038-1-Ig (same clone as 67038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67038-1-Ig (MED30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MED30 Monoclonal antibody (67038-1-Ig)

    WB analysis of HEK-293 using 67038-1-Ig (same clone as 67038-1-PBS)

    WB result of MED30 antibody (67038-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED30 transfected HEK-293 cells. This data was developed using the same antibody clone with 67038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using MED30 Monoclonal antibody (67038-1-Ig)

    WB analysis of pig brain using 67038-1-Ig (same clone as 67038-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67038-1-Ig (MED30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using MED30 Monoclonal antibody (67038-1-Ig)

    WB analysis of rat brain using 67038-1-Ig (same clone as 67038-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67038-1-Ig (MED30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67038-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MED30 Monoclonal antibody, PBS Only

MED30 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67038-1-PBS
Clone No.1D7C4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig

Applications

WB, Indirect ELISA

Synonyms

MED30, mediator complex subunit 30, THRAP6, TRAP25

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67038-1-PBS targets MED30 in WB, Indirect ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MED30 fusion protein Ag10235 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 30
Calculated molecular weight 178 aa, 20 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008226
Gene Symbol MED30
Gene ID (NCBI) 90390
RRIDAB_2882352
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HR3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.