Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, Jurkat cells, HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15338-1-AP targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MED6 fusion protein Ag7541 相同性解析による交差性が予測される生物種 |
| Full Name | mediator complex subunit 6 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28-32 kDa |
| GenBank accession number | BC004106 |
| Gene Symbol | MED6 |
| Gene ID (NCBI) | 10001 |
| RRID | AB_2142554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED6 antibody 15338-1-AP | Download protocol |
| IHC protocol for MED6 antibody 15338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II |