MED6 Polyclonal antibody

MED6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15338-1-AP

Synonyms

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28


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Tested Applications

Positive WB detected inA431 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15338-1-AP targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED6 fusion protein Ag7541 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 6
Calculated molecular weight 28 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC004106
Gene symbol MED6
Gene ID (NCBI) 10001
RRIDAB_2142554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Protocols

Product Specific Protocols
WB protocol for MED6 antibody 15338-1-APDownload protocol
IHC protocol for MED6 antibody 15338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

anh (Verified Customer) (04-18-2022)

claer band on target molecular weight , but sometimes have some unspecific band on membrane other size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse tissue
FH

SITING (Verified Customer) (05-26-2021)

good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED6 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:15338-1-AP)