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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED6 Recombinant antibody (84184-4-RR)

    WB analysis using 84184-4-RR (same clone as 84184-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01087-3

    Sandwich ELISA standard curve of MP01087-3

    Sandwich ELISA standard curve of MP01087-3, Human MED6 Recombinant Matched Antibody Pair - PBS only. 84184-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7541. 84184-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

  • Sample test of MP01087-3

    Sample test of MP01087-3

    The mean MED6 concentration was determined to be 7.45 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 16.00 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 13.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load.

  • Flow cytometry (FC) experiment of A431 cells using MED6 Recombinant antibody (84184-4-RR)

    FC experiment of A431 using 84184-4-RR (same clone as 84184-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01087-3

    Cytometric bead array standard curve of MP01087-3

    Cytometric bead array standard curve of MP01087-3, MED6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84184-4-PBS. Detection antibody: 84184-2-PBS. Standard: Ag7541. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84184-4-RR

    Affinity and Kinetic Characterization of 84184-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

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MED6 Recombinant antibody, PBS Only (Capture)

MED6 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84184-4-PBS
Clone No.241382H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84184-4-PBS targets MED6 as part of a matched antibody pair:

MP01087-3: 84184-4-PBS capture and 84184-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED6 fusion protein Ag7541 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 6
Calculated molecular weight 28 kDa
Observed molecular weight33 kDa
GenBank accession numberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75586
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.