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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MED8 Polyclonal antibody (12182-1-AP)

    WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using MED8 Polyclonal antibody (12182-1-AP)

    WB analysis of mouse liver using 12182-1-AP (same clone as 12182-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using MED8 Polyclonal antibody (12182-1-AP)

    WB analysis of SGC-7901 using 12182-1-AP (same clone as 12182-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MED8 Polyclonal antibody (12182-1-AP)

    IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MED8 Polyclonal antibody (12182-1-AP)

    IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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MED8 Polyclonal antibody, PBS Only

MED8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12182-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12182-1-PBS targets MED8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED8 fusion protein Ag2827 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 8
Calculated molecular weight 212 aa, 23 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC010543
Gene Symbol MED8
Gene ID (NCBI) 112950
RRIDAB_2877834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G25
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.