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  • Featured Product
  • KD/KO Validated

MEF2C Polyclonal antibody, PBS Only

MEF2C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10056-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using MEF2C Polyclonal antibody (10056-1-AP)

    WB analysis of SH-SY5Y using 10056-1-AP (same clone as 10056-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEF2C Polyclonal antibody (10056-1-AP)

    WB analysis using 10056-1-AP (same clone as 10056-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using MEF2C Polyclonal antibody (10056-1-AP)

    WB analysis of mouse colon using 10056-1-AP (same clone as 10056-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using MEF2C Polyclonal antibody (10056-1-AP)

    WB analysis of SH-SY5Y using 10056-1-AP (same clone as 10056-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using MEF2C Polyclonal antibody (10056-1-AP)

    WB analysis of mouse colon using 10056-1-AP (same clone as 10056-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using MEF2C Polyclonal antibody (10056-1-AP)

    WB analysis of mouse colon using 10056-1-AP (same clone as 10056-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using MEF2C Polyclonal antibody (10056-1-AP)

    IP experiment of SH-SY5Y using 10056-1-AP (same clone as 10056-1-PBS)

    IP result of anti-MEF2C (IP:10056-1-AP, 3ug; Detection:10056-1-AP 1:500) with SH-SY5Y cells lysate 2000ug. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using MEF2C Polyclonal antibody (10056-1-AP)

    IP experiment of SH-SY5Y using 10056-1-AP (same clone as 10056-1-PBS)

    IP result of anti-MEF2C (IP:10056-1-AP, 3ug; Detection:10056-1-AP 1:500) with SH-SY5Y cells lysate 2000ug. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment using MEF2C Polyclonal antibody (10056-1-AP)

    IP experiment using 10056-1-AP (same clone as 10056-1-PBS)

    IP result of anti-MEF2C (10056-1-AP for IP and Detection) with SHSY5Y cell. This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MEF2C Polyclonal antibody (10056-1-AP)

    IHC staining of human lymphoma using 10056-1-AP (same clone as 10056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10056-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MEF2C Polyclonal antibody (10056-1-AP)

    IF Staining of HepG2 using 10056-1-AP (same clone as 10056-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MEF2C antibody (10056-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MEF2C Polyclonal antibody (10056-1-AP)

    FC experiment of HeLa using 10056-1-AP (same clone as 10056-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MEF2C (10056-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10056-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10056-1-PBS targets MEF2C in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag0020 相同性解析による交差性が予測される生物種
Full Name myocyte enhancer factor 2C
Calculated molecular weight 51 kDa
Observed molecular weight45-70 kDa
GenBank accession numberBC156603
Gene symbol MEF2C
Gene ID (NCBI) 4208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.