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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16953-1-AP

Synonyms

myocyte enhancer factor 2C

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using MEF2C Polyclonal antibody (16953-1-AP)

    WB analysis of human heart using 16953-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TF-1 cells using MEF2C Polyclonal antibody (16953-1-AP)

    WB analysis of TF-1 using 16953-1-AP

    TF-1 cells were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEF2C Polyclonal antibody (16953-1-AP)

    WB analysis of HeLa using 16953-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MEF2C Polyclonal antibody (16953-1-AP)

    WB analysis of mouse skeletal muscle using 16953-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman heart tissue, HeLa cells, mouse skeletal muscle tissue, TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16953-1-AP targets MEF2C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag10583 相同性解析による交差性が予測される生物種
Full Name myocyte enhancer factor 2C
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight45-70 kDa
GenBank accession numberBC026341
Gene symbol MEF2C
Gene ID (NCBI) 4208
RRIDAB_1851364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

Protocols

Product Specific Protocols
WB protocol for MEF2C antibody 16953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols