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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEF2C Polyclonal antibody, PBS Only

MEF2C Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20326-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of HeLa using 20326-1-AP (same clone as 20326-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of SH-SY5Y using 20326-1-AP (same clone as 20326-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of mouse testis using 20326-1-AP (same clone as 20326-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of mouse small intestine using 20326-1-AP (same clone as 20326-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lymph tissue using MEF2C Polyclonal antibody (20326-1-AP)

    WB analysis of rat lymph using 20326-1-AP (same clone as 20326-1-PBS)

    rat lymph tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IHC staining of human heart using 20326-1-AP (same clone as 20326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IHC staining of human heart using 20326-1-AP (same clone as 20326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IF Staining of mouse brain using 20326-1-AP (same clone as 20326-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MEF2C Polyclonal antibody (20326-1-AP)

    IF Staining of mouse brain using 20326-1-AP (same clone as 20326-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20326-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20326-1-PBS targets MEF2C in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag13255 相同性解析による交差性が予測される生物種
Full Name myocyte enhancer factor 2C
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight45-70 kDa
GenBank accession numberBC026341
Gene symbol MEF2C
Gene ID (NCBI) 4208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.