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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEI1 Polyclonal antibody

MEI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13456-1-AP

Synonyms

MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MEI1 Polyclonal antibody (13456-1-AP)

    WB analysis of mouse brain using 13456-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13456-1-AP (MEI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MEI1 Polyclonal antibody (13456-1-AP)

    IHC staining of human cervical cancer using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MEI1 Polyclonal antibody (13456-1-AP)

    IHC staining of human cervical cancer using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using MEI1 Polyclonal antibody (13456-1-AP)

    IHC staining of rat testis using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using MEI1 Polyclonal antibody (13456-1-AP)

    IHC staining of rat testis using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using MEI1 Polyclonal antibody (13456-1-AP)

    IHC staining of human urothelial carcinoma using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using MEI1 Polyclonal antibody (13456-1-AP)

    IHC staining of human urothelial carcinoma using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman cervical cancer tissue, human urothelial carcinoma tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13456-1-AP targets MEI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEI1 fusion protein Ag4253 相同性解析による交差性が予測される生物種
Full Name meiosis inhibitor 1
Calculated molecular weight 894 aa, 99 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC032248
Gene symbol MEI1
Gene ID (NCBI) 150365
RRIDAB_2877951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEI1 may play a role in meiosis during spermatogenesis. It is required for normal meiotic chromosome synapsis. MEI1 may be involved in the formation of meiotic double-strand breaks (DSBs) in spermatocytes. This antibody recognizes isoform 1, 2, 3, 5, and 7 of MEI1 gene with MW 141 kDa, 67-72 kDa,49-54 kDa and 26 kDa .

Protocols

Product Specific Protocols
WB protocol for MEI1 antibody 13456-1-APDownload protocol
IHC protocol for MEI1 antibody 13456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols