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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEI4 Polyclonal antibody

MEI4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29892-1-AP

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MEI4 Polyclonal antibody (29892-1-AP)

    WB analysis using 29892-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 29892-1-AP (MEI4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MEI4 Polyclonal antibody (29892-1-AP)

    IHC staining of mouse testis using 29892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MEI4 Polyclonal antibody (29892-1-AP)

    IHC staining of mouse testis using 29892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using MEI4 Polyclonal antibody (29892-1-AP)

    IF Staining of mouse testis using 29892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MEI4 antibody (29892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29892-1-AP targets MEI4 in WB, IHC, IF-P, ELISA applications and shows reactivity with Mouse, human samples.

Tested Reactivity Mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEI4 fusion protein Ag31859 相同性解析による交差性が予測される生物種
Full Name Meiosis-specific protein MEI4
Calculated molecular weight44 kDa
Observed molecular weight45 kDa
GenBank accession numberNM_001322247.1
Gene symbol MEI4
Gene ID (NCBI) 101928601
RRIDAB_3086182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MEI4 antibody 29892-1-APDownload protocol
IHC protocol for MEI4 antibody 29892-1-APDownload protocol
IF protocol for MEI4 antibody 29892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols