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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEIS2 Polyclonal antibody

MEIS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11550-1-AP

Synonyms

Homeobox protein Meis2, HsT18361, Meis homeobox 2, Meis1 related protein 1, MEIS2, MRG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEIS2 Polyclonal antibody (11550-1-AP)

    WB analysis using 11550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MEIS2 Polyclonal antibody (11550-1-AP)

    WB analysis using 11550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MEIS2 Polyclonal antibody (11550-1-AP)

    WB analysis of PC-3 using 11550-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEIS2 Polyclonal antibody (11550-1-AP)

    IHC staining of human prostate cancer using 11550-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEIS2 Polyclonal antibody (11550-1-AP)

    IHC staining of human prostate cancer using 11550-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, PC-3 cells, A549 cells, LNCaP cells, HCT 116 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11550-1-AP targets MEIS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEIS2 fusion protein Ag2096 相同性解析による交差性が予測される生物種
Full Name Meis homeobox 2
Calculated molecular weight 381 aa, 42 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC001516
Gene symbol MEIS2
Gene ID (NCBI) 4212
RRIDAB_2143028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEIS2, also named as Meis1-related protein 1 or MRG1, is a 477 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the TALE/MEIS homeobox family. MEIS2 is expressed in various tissues and at high level in the lymphoid organs of hematopoietic tissues. MEIS2 localizes in the nucleus and is involved in transcriptional regulation. MEIS2 is a critical downstream effector within an essential homeoprotein network that serving a rate-limiting regulatory role in MLL leukemogenesis. MEIS2 as midbrain specific marker is upregulated concomitantly with the morphological transformation of hindbrain to midbrain.

Protocols

Product Specific Protocols
WB protocol for MEIS2 antibody 11550-1-APDownload protocol
IHC protocol for MEIS2 antibody 11550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Proc Natl Acad Sci U S A

Congenital diaphragmatic hernia candidate genes derived from embryonic transcriptomes.

Authors - Russell Meaghan K MK
View Article
mouseWB

Aging Cell

Myeloid ectopic viral integration site 2 accelerates the progression of Alzheimer's disease

Authors - Yuting Cui
  • KD Validated
View Article