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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of A431 using 67872-1-Ig (same clone as 67872-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (MEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

    Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

    WB result of MEK1 antibody (67872-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEK1 transfected HeLa cells. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of NIH/3T3 using 67872-1-Ig (same clone as 67872-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of HSC-T6 using 67872-1-Ig (same clone as 67872-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of PC-3 using 67872-1-Ig (same clone as 67872-1-PBS)

    Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50180-1

    Cytometric bead array in cell lysate using MP50180-1

    Cytometric bead array in cell lysate using MP50180-1, Phospho-MEK1 (Thr386) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67872-1-PBS. Detection antibody: 68015-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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  • Featured Product
  • KD/KO Validated

MEK1 Monoclonal antibody, PBS Only (Capture)

MEK1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67872-1-PBS
Clone No.3F6D7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

MAP2K1, MAPKK 1, MAP2K 1, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67872-1-PBS targets MEK1 as part of a matched antibody pair:

MP50180-1: 67872-1-PBS capture and 68015-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 1
Calculated molecular weight 43 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC139729
Gene Symbol MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
RRIDAB_2918630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02750
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.