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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEK2 Monoclonal antibody, PBS Only

MEK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H8C2

Cat no : 67410-1-PBS

Synonyms

MAP2K2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MEK2 Monoclonal antibody (67410-1-Ig)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MAP2K2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEK2 Monoclonal antibody (67410-1-Ig)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEK2 Monoclonal antibody (67410-1-Ig)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MAP2K2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MEK2 Monoclonal antibody (67410-1-Ig)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MAP2K2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MEK2 Monoclonal antibody (67410-1-Ig)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MAP2K2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEK2 Monoclonal antibody (67410-1-Ig)

    IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67410-1-PBS targets MEK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MEK2 fusion protein Ag29871 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC018645
Gene symbol MEK2
Gene ID (NCBI) 5605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.