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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEK1/2 Monoclonal antibody (67410-1-Ig)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEK1/2 Monoclonal antibody (67410-1-Ig)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEK1/2 Monoclonal antibody (67410-1-Ig)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MEK1/2 Monoclonal antibody (67410-1-Ig)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MEK1/2 Monoclonal antibody (67410-1-Ig)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEK1/2 Monoclonal antibody (67410-1-Ig)

    IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK1/2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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MEK1/2 Monoclonal antibody, PBS Only

MEK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67410-1-PBS
Clone No.1H8C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67410-1-PBS targets MEK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MEK1/2 fusion protein Ag29871 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC018645
Gene Symbol MEK2
Gene ID (NCBI) 5605
RRIDAB_2882651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36507
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 67410-1-Ig can recognize both the MEK2 and MEK1.