Validation Data Gallery

Filter: AllWBIHC

WB analysis of Jurkat using 11403-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 11403-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11403-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MELK Polyclonal antibody

MELK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11403-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, K-562 cells
Positive IHC detected in	human breast cancer tissue, mouse colon tissue, mouse kidney tissue, human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 5 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

11403-1-AP targets MELK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MELK fusion protein Ag1897 相同性解析による交差性が予測される生物種
Full Name	maternal embryonic leucine zipper kinase
Calculated molecular weight	651 aa, 75 kDa
Observed molecular weight	50-70 kDa
GenBank accession number	BC014039
Gene Symbol	MELK
Gene ID (NCBI)	9833
RRID	AB_2877762
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14680
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Protocols

Product Specific Protocols
WB protocol for MELK antibody 11403-1-AP	Download protocol
IHC protocol for MELK antibody 11403-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	Int J Oncol MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway. Authors - Jiecheng Ye View Article
human	IHC	Med Sci Monit Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population. Authors - Shaohan Wu View Article
human	IHC	Drug Des Devel Ther A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK Authors - Yan Ma View Article
human	WB,IHC	J Cancer Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma Authors - Yan Wang View Article
human	WB,IHC	Cancer Cell Int Upregulation of MELK promotes chemoresistance and induces macrophage M2 polarization via CSF-1/JAK2/STAT3 pathway in gastric cancer Authors - Pengfei Su View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11403-1-AP

WB analysis using 11403-1-AP

WB analysis of K-562 using 11403-1-AP

IHC staining of human breast cancer using 11403-1-AP

IHC staining of human breast cancer using 11403-1-AP

IHC staining of human colon cancer using 11403-1-AP

IHC staining of mouse colon using 11403-1-AP

IHC staining of mouse colon using 11403-1-AP

IHC staining of mouse colon using 11403-1-AP

IHC staining of mouse kidney using 11403-1-AP

IHC staining of mouse kidney using 11403-1-AP

IHC staining of human lung cancer using 11403-1-AP

IHC staining of human lung cancer using 11403-1-AP

IHC staining of human colon cancer using 11403-1-AP

MELK Polyclonal antibody

MELK Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway.

Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population.

A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Upregulation of MELK promotes chemoresistance and induces macrophage M2 polarization via CSF-1/JAK2/STAT3 pathway in gastric cancer