MELK Polyclonal antibody

Name: MELK Polyclonal antibody
Brand: Proteintech
SKU: 11403-1-AP

MELK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11403-1-AP

Synonyms

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of Jurkat using 11403-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 11403-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11403-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11403-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11403-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, K-562 cells
Positive IHC detected in	human breast cancer tissue, mouse colon tissue, mouse kidney tissue, human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 4 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

11403-1-AP targets MELK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MELK fusion protein Ag1897 相同性解析による交差性が予測される生物種
Full Name	maternal embryonic leucine zipper kinase
Calculated molecular weight	651 aa, 75 kDa
Observed molecular weight	50-70 kDa
GenBank accession number	BC014039
Gene symbol	MELK
Gene ID (NCBI)	9833
RRID	AB_2877762
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Protocols

Product Specific Protocols
WB protocol for MELK antibody 11403-1-AP	Download protocol
IHC protocol for MELK antibody 11403-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	Int J Oncol MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway. Authors - Jiecheng Ye View Article
human	IHC	Med Sci Monit Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population. Authors - Shaohan Wu View Article
human	IHC	Drug Des Devel Ther A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK Authors - Yan Ma View Article
human	WB,IHC	J Cancer Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma Authors - Yan Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MELK Polyclonal antibody

MELK Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of Jurkat using 11403-1-AP

WB analysis using 11403-1-AP

WB analysis of K-562 using 11403-1-AP

IHC staining of human breast cancer using 11403-1-AP

IHC staining of human breast cancer using 11403-1-AP

IHC staining of human colon cancer using 11403-1-AP

IHC staining of mouse colon using 11403-1-AP

IHC staining of mouse colon using 11403-1-AP

IHC staining of mouse colon using 11403-1-AP

IHC staining of mouse kidney using 11403-1-AP

IHC staining of mouse kidney using 11403-1-AP

IHC staining of human lung cancer using 11403-1-AP

IHC staining of human lung cancer using 11403-1-AP

IHC staining of human colon cancer using 11403-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway.

Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population.

A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma