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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MELK Recombinant antibody

MELK Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230106D4

Cat no : 82893-1-RR

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using MELK Recombinant antibody (82893-1-RR)

    FC experiment of MCF-7 using 82893-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82893-1-RR

    Affinity and Kinetic Characterization of 82893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82893-1-RR targets MELK in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MELK fusion protein Ag1897 相同性解析による交差性が予測される生物種
Full Name maternal embryonic leucine zipper kinase
Calculated molecular weight 651 aa, 75 kDa
GenBank accession numberBC014039
Gene symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Protocols

Product Specific Protocols
FC protocol for MELK antibody 82893-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols