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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MELK Recombinant antibody, PBS Only (Capture/Detector)

MELK Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230106A4

Cat no : 82893-2-PBS

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MELK Recombinant antibody (82893-2-RR)

    WB analysis using 82893-2-RR (same clone as 82893-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00075-4

    Sandwich ELISA standard curve of MP00075-4

    Sandwich ELISA standard curve of MP00075-4, Human MELK Recombinant Matched Antibody Pair - PBS only. 82893-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1897. 82893-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00075-4

    Sample test of MP00075-4

    NCCIT and K-562 cell lysate were measured. The human MELK concentration of detected samples was determined to be 15.9 ng/mL (based on a 4.5 mg/mL extract load) in NCCIT cell lysate and 16.4 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MELK Recombinant antibody (82893-2-RR)

    IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MELK Recombinant antibody (82893-2-RR)

    IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00075-1

    Cytometric bead array standard curve of MP00075-1

    Cytometric bead array standard curve of MP00075-1, MELK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82893-2-PBS. Detection antibody: 82893-3-PBS. Standard: Ag1897. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP00075-2

    Cytometric bead array standard curve of MP00075-2

    Cytometric bead array standard curve of MP00075-2, MELK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82893-4-PBS. Detection antibody: 82893-2-PBS. Standard: Ag1897. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 82893-2-RR

    Affinity and Kinetic Characterization of 82893-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82893-2-PBS targets MELK as part of a matched antibody pair:

MP00075-1: 82893-2-PBS capture and 82893-3-PBS detection (validated in Cytometric bead array)

MP00075-2: 82893-4-PBS capture and 82893-2-PBS detection (validated in Cytometric bead array)

MP00075-4: 82893-2-PBS capture and 82893-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MELK fusion protein Ag1897 相同性解析による交差性が予測される生物種
Full Name maternal embryonic leucine zipper kinase
Calculated molecular weight 651 aa, 75 kDa
Observed molecular weight50-70 kDa
GenBank accession numberBC014039
Gene symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.