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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEN1 Monoclonal antibody, PBS Only

MEN1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A6A11

Cat no : 68473-1-PBS

Synonyms

SCG2, Menin 1, Menin, MEAI, 3A6A11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEN1 Monoclonal antibody (68473-1-Ig)

    WB analysis using 68473-1-Ig (same clone as 68473-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68473-1-Ig (MEN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68473-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MEN1 Monoclonal antibody (68473-1-Ig)

    IHC staining of mouse brain using 68473-1-Ig (same clone as 68473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68473-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MEN1 Monoclonal antibody (68473-1-Ig)

    IHC staining of mouse brain using 68473-1-Ig (same clone as 68473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68473-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68473-1-PBS targets MEN1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MEN1 fusion protein Ag7566 相同性解析による交差性が予測される生物種
Full Name multiple endocrine neoplasia I
Calculated molecular weight 68 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC002544
Gene symbol MEN1
Gene ID (NCBI) 4221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. It is located in nucleus and ubiquitous expression in spleen and lymph node. The calculated molecular weight of MEN1 is 68 kDa. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice.