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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of HepG2 using 18804-1-AP (same clone as 18804-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of HeLa using 18804-1-AP (same clone as 18804-1-PBS)

    WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of A375 using 18804-1-AP (same clone as 18804-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of MCF-7 using 18804-1-AP (same clone as 18804-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of HeLa using 18804-1-AP (same clone as 18804-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of A2780 using 18804-1-AP (same clone as 18804-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MEPE Polyclonal antibody, PBS Only

MEPE Polyclonal Antibody for WB, Indirect ELISA
Cat No. 18804-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18804-1-PBS targets MEPE in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name matrix extracellular phosphoglycoprotein
Calculated molecular weight 525 aa, 58 kDa
Observed molecular weight 43 kDa, 98 kDa
GenBank accession numberBC128158
Gene Symbol MEPE
Gene ID (NCBI) 56955
RRIDAB_2143574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ76
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).