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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MEPE Polyclonal antibody (23256-1-AP)

    WB analysis of HeLa using 23256-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MEPE Polyclonal antibody (23256-1-AP)

    WB analysis of MCF-7 using 23256-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MEPE Polyclonal antibody (23256-1-AP)

    WB analysis of MCF-7 using 23256-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MEPE Polyclonal antibody (23256-1-AP)

    IP experiment of HeLa using 23256-1-AP

    IP result of anti-MEPE (IP:23256-1-AP, 4ug; Detection:23256-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung tissue using MEPE Polyclonal antibody (23256-1-AP)

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MEPE Polyclonal antibody (23256-1-AP)

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MEPE Polyclonal antibody (23256-1-AP)

    IF Staining of HepG2 using 23256-1-AP

    Immunofluorescent analysis of HepG2 cells, using MEPE antibody 23256-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Cat No. 23256-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Synonyms

MEPE, OF45, Osteoblast/osteocyte factor 45

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23256-1-AP targets MEPE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEPE fusion protein Ag19758 相同性解析による交差性が予測される生物種
Full Name matrix extracellular phosphoglycoprotein
Calculated molecular weight 525 aa, 58 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC128158
Gene Symbol MEPE
Gene ID (NCBI) 56955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.

Protocols

Product Specific Protocols
WB protocol for MEPE antibody 23256-1-APDownload protocol
IHC protocol for MEPE antibody 23256-1-APDownload protocol
IF protocol for MEPE antibody 23256-1-APDownload protocol
IP protocol for MEPE antibody 23256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols