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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MESDC2 Polyclonal antibody

MESDC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10958-1-AP

Synonyms

MESDM, MESD, LRP chaperone MESD, LDLR chaperone MESD, KIAA0081

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MESDC2 Polyclonal antibody (10958-1-AP)

    WB analysis using 10958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HEK-293 cells using MESDC2 Polyclonal antibody (10958-1-AP)

    WB analysis of HEK-293 using 10958-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MESDC2 Polyclonal antibody (10958-1-AP)

    WB analysis of HeLa using 10958-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MESDC2 Polyclonal antibody (10958-1-AP)

    IHC staining of human placenta using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MESDC2 Polyclonal antibody (10958-1-AP)

    IHC staining of human placenta using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MESDC2 Polyclonal antibody (10958-1-AP)

    IHC staining of human stomach using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MESDC2 Polyclonal antibody (10958-1-AP)

    IHC staining of human stomach using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MESDC2 Polyclonal antibody (10958-1-AP)

    IHC staining of human stomach using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10958-1-AP targets MESDC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MESDC2 fusion protein Ag1403 相同性解析による交差性が予測される生物種
Full Name mesoderm development candidate 2
Calculated molecular weight 26 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC009210
Gene symbol MESDC2
Gene ID (NCBI) 23184
RRIDAB_2143722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MESDC2 antibody 10958-1-APDownload protocol
IHC protocol for MESDC2 antibody 10958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Parijat (Verified Customer) (09-20-2023)

Works well in 293T cells

  • Applications: Western Blot