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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MESDC2 Monoclonal antibody

MESDC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2E7

Cat no : 67441-1-Ig

Synonyms

BOCA, KIAA0081, LDLR chaperone MESD, MESD, MESDC2, Mesoderm development protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MESDC2 Monoclonal antibody (67441-1-Ig)

    WB analysis using 67441-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MESDC2 Monoclonal antibody (67441-1-Ig)

    WB analysis of human placenta using 67441-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MESDC2 Monoclonal antibody (67441-1-Ig)

    IHC staining of human placenta using 67441-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MESDC2 Monoclonal antibody (67441-1-Ig)

    IHC staining of human placenta using 67441-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MESDC2 Monoclonal antibody (67441-1-Ig)

    IF Staining of HEK-293 using 67441-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MESDC2 antibody (67441-1-Ig, Clone: 1A2E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inJAR cells, human placenta tissue, HeLa cells, A549 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67441-1-Ig targets MESDC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MESDC2 fusion protein Ag1403 相同性解析による交差性が予測される生物種
Full Name mesoderm development candidate 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC009210
Gene symbol MESDC2
Gene ID (NCBI) 23184
RRIDAB_2882677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MESDC2 antibody 67441-1-IgDownload protocol
IHC protocol for MESDC2 antibody 67441-1-IgDownload protocol
IF protocol for MESDC2 antibody 67441-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols