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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEST Monoclonal antibody, PBS Only

MEST Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4A1

Cat no : 60263-1-PBS

Synonyms

MEST, PEG1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using MEST Monoclonal antibody (60263-1-Ig)

    WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEST Monoclonal antibody (60263-1-Ig)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEST Monoclonal antibody (60263-1-Ig)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using MEST Monoclonal antibody (60263-1-Ig)

    IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60263-1-PBS targets MEST in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MEST fusion protein Ag11929 相同性解析による交差性が予測される生物種
Full Name mesoderm specific transcript homolog (mouse)
Calculated molecular weight 38 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC011908
Gene symbol MEST
Gene ID (NCBI) 4232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.