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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using MEST Monoclonal antibody (60263-1-Ig)

    WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEST Monoclonal antibody (60263-1-Ig)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEST Monoclonal antibody (60263-1-Ig)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using MEST Monoclonal antibody (60263-1-Ig)

    IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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MEST Monoclonal antibody, PBS Only

MEST Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60263-1-PBS
Clone No.1A4A1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60263-1-PBS targets MEST in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MEST fusion protein Ag11929 相同性解析による交差性が予測される生物種
Full Name mesoderm specific transcript homolog (mouse)
Calculated molecular weight 38 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC011908
Gene Symbol MEST
Gene ID (NCBI) 4232
RRIDAB_2881384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5EB52
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.