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  • Featured Product
  • KD/KO Validated

c-Met (Cytoplasmic) Polyclonal antibody

c-Met (Cytoplasmic) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25869-1-AP

Synonyms

c-Met, MET, HGF/SF receptor, HGF receptor, EC:2.7.10.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    WB analysis using 25869-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    WB analysis of HepG2 using 25869-1-AP

    WB result of c-Met antibody (25869-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met transfected HepG2 cells.

  • Western Blot (WB) analysis of MDCK cells using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    WB analysis of MDCK using 25869-1-AP

    MDCK cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    WB analysis of A431 using 25869-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IP experiment of HeLa using 25869-1-AP

    IP result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human lung cancer using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human lung cancer using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human breast cancer using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human breast cancer using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human colon using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human colon using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human liver cancer using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IHC staining of human liver cancer using 25869-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    IF Staining of HepG2 using 25869-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using c-Met (Cytoplasmic) antibody (25869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using c-Met (Cytoplasmic) Polyclonal antibody (25869-1-AP)

    FC experiment of HeLa using 25869-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-Met (Cytoplasmic) (25869-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse liver tissue, MDCK cells, HepG2 cells, A431 cells, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25869-1-AP targets c-Met (Cytoplasmic) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-Met (Cytoplasmic) fusion protein Ag23140 相同性解析による交差性が予測される生物種
Full Name met proto-oncogene (hepatocyte growth factor receptor)
Calculated molecular weight 1390 aa, 155 kDa
Observed molecular weight 145 kDa
GenBank accession numberBC130420
Gene symbol MET
Gene ID (NCBI) 4233
RRIDAB_2880276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Protocols

Product Specific Protocols
WB protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
IHC protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
IF protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
IP protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Metab

LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration

Authors - Yalan Deng
View Article
ratWB

Acta Pharmacol Sin

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Authors - Ji-Chao Wu
View Article
mouseWB

Clin Transl Med

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Authors - Xi Dong
View Article
WB

Oxid Med Cell Longev

PMID: 31781338

Authors - Ya-Dong Liu
View Article
humanIHC

Mol Ther Nucleic Acids

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Authors - Yalei Cui
View Article
humanWB

Mol Ther Nucleic Acids

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Authors - Yalei Cui
View Article