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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METAP2 Polyclonal antibody

METAP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17040-1-AP

Synonyms

MAP 2, MAP2, MetAP 2, METAP2, Methionine aminopeptidase 2, methionyl aminopeptidase 2, MNPEP, p67, p67eIF2, Peptidase M 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METAP2 Polyclonal antibody (17040-1-AP)

    WB analysis using 17040-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using METAP2 Polyclonal antibody (17040-1-AP)

    WB analysis of MCF-7 using 17040-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using METAP2 Polyclonal antibody (17040-1-AP)

    WB analysis of HepG2 using 17040-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using METAP2 Polyclonal antibody (17040-1-AP)

    WB analysis of MCF-7 using 17040-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using METAP2 Polyclonal antibody (17040-1-AP)

    WB analysis using 17040-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using METAP2 Polyclonal antibody (17040-1-AP)

    IP experiment of MCF-7 using 17040-1-AP

    IP result of anti-METAP2 (IP:17040-1-AP, 4ug; Detection:17040-1-AP 1:300) with MCF-7 cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using METAP2 Polyclonal antibody (17040-1-AP)

    IF Staining of HepG2 using 17040-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17040-1-AP (METAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, mouse pancreas tissue, mouse testis tissue
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17040-1-AP targets METAP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METAP2 fusion protein Ag10564 相同性解析による交差性が予測される生物種
Full Name methionyl aminopeptidase 2
Calculated molecular weight 478 aa, 53 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC013782
Gene symbol METAP2
Gene ID (NCBI) 10988
RRIDAB_2144162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MetAP2, also known as MAP2, P67EIF2 or p67, is one of the three known methionine aminopeptidases responsible for the removal of the N-terminal translation initiator methionine from newly synthesized proteins, which is a critical step in protein maturation (PMID: 27692546, 16568094). The enzyme binds two cobalt or manganese ions in its active site (PMID: 11524009, 12718546). As higher MetAP2 expression was observed in tumor (mesothelioma, neuroblastoma, and colorectal carcinoma) cells compared with normal cells, the enzyme serves as a target for anti-angiogenic compounds of natural origin, such as fumagillin and ovalicin (PMID: 11485930, 15927358, 15102683, 9224570). Selective inhibition of MetAP2 stops vascularization and tumor growth in animal models (PMID: 12118091). MetAP2 has 3 isoforms with molecular mass of 50-53 kDa and apparent molecular mass 67 kD.

Protocols

Product Specific Protocols
WB protocol for METAP2 antibody 17040-1-APDownload protocol
IF protocol for METAP2 antibody 17040-1-APDownload protocol
IP protocol for METAP2 antibody 17040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Decoding the Function of Expansion Segments in Ribosomes.

Authors - Kotaro Fujii
View Article