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  • Featured Product
  • KD/KO Validated

METTL1 Polyclonal antibody, PBS Only

METTL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14994-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL1 Polyclonal antibody (14994-1-AP)

    WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using METTL1 Polyclonal antibody (14994-1-AP)

    WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A2780 cells using METTL1 Polyclonal antibody (14994-1-AP)

    IP experiment of A2780 using 14994-1-AP (same clone as 14994-1-PBS)

    IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL1 Polyclonal antibody (14994-1-AP)

    IHC staining of human liver cancer using 14994-1-AP (same clone as 14994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14994-1-AP (METTL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using METTL1 Polyclonal antibody (14994-1-AP)

    IF Staining of HepG2 using 14994-1-AP (same clone as 14994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14994-1-PBS targets METTL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL1 fusion protein Ag6980 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC000550
Gene symbol METTL1
Gene ID (NCBI) 4234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.