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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL1 Polyclonal antibody (14994-1-AP)

    WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using METTL1 Polyclonal antibody (14994-1-AP)

    WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A2780 cells using METTL1 Polyclonal antibody (14994-1-AP)

    IP experiment of A2780 using 14994-1-AP (same clone as 14994-1-PBS)

    IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL1 Polyclonal antibody (14994-1-AP)

    IHC staining of human liver cancer using 14994-1-AP (same clone as 14994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14994-1-AP (METTL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using METTL1 Polyclonal antibody (14994-1-AP)

    IF Staining of HepG2 using 14994-1-AP (same clone as 14994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

METTL1 Polyclonal antibody, PBS Only

METTL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14994-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14994-1-PBS targets METTL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL1 fusion protein Ag6980 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC000550
Gene Symbol METTL1
Gene ID (NCBI) 4234
RRIDAB_2142013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBP6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.