METTL1 Recombinant antibody
METTL1 Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
241108G8
Cat no : 84272-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Caco-2 cells, HuH-7 cells, mouse brain tissue |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 84272-1-RR is METTL1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | METTL1 fusion protein Ag7055 相同性解析による交差性が予測される生物種 |
Full Name | methyltransferase like 1 |
Calculated molecular weight | 31 kDa |
Observed molecular weight | 31-35 kDa |
GenBank accession number | BC000550 |
Gene symbol | METTL1 |
Gene ID (NCBI) | 4234 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.
Protocols
Product Specific Protocols | |
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WB protocol for METTL1 antibody 84272-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |