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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL14 Recombinant antibody (80790-1-RR)

    WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80790-1-RR (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using METTL14 Recombinant antibody (80790-1-RR)

    WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB result of METTL14 antibody (80790-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected HeLa cells. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using METTL14 Recombinant antibody (80790-1-RR)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using METTL14 Recombinant antibody (80790-1-RR)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using METTL14 Recombinant antibody (80790-1-RR)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80790-1-RR

    Affinity and Kinetic Characterization of 80790-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80790-1-RR against Human METTL14 were performed. The affinity constant is 9.12 nM.

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  • Featured Product
  • KD/KO Validated

METTL14 Recombinant antibody, PBS Only

METTL14 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80790-1-PBS
Clone No.2E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80790-1-PBS targets METTL14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL14 fusion protein Ag14325 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 14
Calculated molecular weight 456 aa, 52 kDa
Observed molecular weight55-60 kDa
GenBank accession numberBC007449
Gene Symbol METTL14
Gene ID (NCBI) 57721
RRIDAB_2918912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCE5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.